علوم زیستی دریا
Hassan Zadabbas Shahabadi; Arash Akbarzadeh; Hamideh Ofoghi; Sabihe Soleimanian-Zad; Saeid Kadkhodaei
Abstract
In modern aquaculture, energy consumption, raw materials used, and environmental effects should be considered, therefore, during the last decade, phytase has been used by aquatic feed industries as an enzyme supplement to increase the digestibility and absorption of plant compounds and improve growth ...
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In modern aquaculture, energy consumption, raw materials used, and environmental effects should be considered, therefore, during the last decade, phytase has been used by aquatic feed industries as an enzyme supplement to increase the digestibility and absorption of plant compounds and improve growth performance along with reducing phosphorus pollution in the aquatic environment. In this study, by using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, the bacterial phytase gene was inserted into the model microalgae Chlamydomonas reinhardtii in the designed position of the NR gene, and the correct editing was checked and confirmed by PCR. Confirmation of the efficiency and expression of the phytase gene placed in the NR site was investigated using SDS Page gel. Also, the translation of the phytase gene was confirmed by SDS PAGE on five colonies containing the correct editing and one control sample colony as confirmation of the expression of the transgene inserted at the protein level, which indicated the successful transcription and translation of the phytase gene inserted in the exon 2 position of the microalgae NR gene. considering the benefits of producing and using phytase enzymes in aquatic diets, reducing production costs and eutrophication of aquatic ecosystems will be achieved and it can be a new perspective to accelerate the commercial production of other recombinant proteins in organisms with nutritional value "C. reinhardtii" and provide the development of the fish feed industry and modern aquaculture.
علوم زیستی دریا
Mahdieh Ahmadi; Arash Akbarzadeh
Abstract
In this study, the structure of fish populations of Saurida tumbil in the waters of Hormozgan province was studied using morphometric and meristic characteristics. Samples were obtained from three parts of Bandar Lengeh, Hormoz Island and Minab and 22 morphometric characteristics and 5 meristic characteristics ...
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In this study, the structure of fish populations of Saurida tumbil in the waters of Hormozgan province was studied using morphometric and meristic characteristics. Samples were obtained from three parts of Bandar Lengeh, Hormoz Island and Minab and 22 morphometric characteristics and 5 meristic characteristics were measured in each fish .Morphometric characteristics were standardized before the analysis to reduce the errors caused by alometric growth. The results of one-way ANOVA showed that 9 morphometric characteristics were significantly different among the samples in different regions, also, there were no significant statistical differences between the specimens in the 5 meristic characteristics. The results obtained from the distinguishing functions 1 and 2 for the morphometric characteristics showed a high degree of differentiation among Saurida tumbil stocks in the three studied regions. While there was little difference about the meristic characteristics and the samples areas were highly overlapping in the three study. On the other hand, the percentages of people who were correctly placed in their main population were for morphometric and meristic characteristics, 94.3% and 49.5% respectively. The results of this study showed that S.tumbil differ in terms of morphometric features and it is likely that there exist different stocks of this species in Iranian coasts of the Persian Gulf in Hormozgan that should be considered for fisheries management.